GROWTH AND MULTIPLICATION OF ORCHID BUDS IN VITRO WITH THE ADDITION OF CORN (Zea mays) AND TOMATO (Licopersicum esculentum mill) EXTRACT

Abstract

Vanda Foetida orchid is a popular ornamental plant because of its beautiful flowers. Propagation of Vanda orchids by tissue culture method can be done to facilitate the supply of plant seeds. This study aims to determine the concentration of corn and tomato extracts and their interactions to produce growth and multiplication of Vanda orchid buds. The study was conducted at the Jember Polytechnic Culture Laboratory. This study used factorial Completely Randomized Design, the first factor was tomato extract with a concentration of 100 ml / L, 125 ml / L, 150 ml / L and the second factor was corn extract with a concentration of 50 g / L, 100 g / L, 150 g / L with 3 replications. Observation variables included plant height, number of leaves, number of shoots, number of roots and width of leaves. Data was analyzed by Analysis of Variants and if significantly different continued with LSD test at the level of 5%. The results of the study showed that the use of corn and tomato extracts had not been able to significantly influence the improvement of all observed parameters in Vanda Foetida Orchids.