GHR mRNA Expression in the Liver and Muscles of Local Pigs from The Islands in North Sulawesi, Indonesia
Abstract
The GHR gene is known as a member of the growth gene's superfamily that function important in controlling the growth. This study was designed to investigate the expression of GHR genes in the liver and muscles of local pig from the islands in North Sulawesi, Indonesia. The parameters measured was GHR mRNA expression by reverse tranriptase RT-PCR. mRNA sample was taken from liver and longisimus dorsi muscle of sacrifice pigs and cDNA was amplified using specific primer for target genes. The GHR primer gene used was 5’ TTT TCT GGG AGT GAA GCC AC 3' and R = 5’ AGG GCT CTG TAA ACC GTG AC 3' with its housekeeping gene GAPDH. The result showed that GHR is expressed in the liver and muscles of local pig from the islands in North Sulawesi. GHR mRNA expression in muscles was higher than in liver (P>0.05). The GHR gene expression was also regulated by factors such as nutritional intake, GH, ages, temperature and hormones.
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